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Specimen Preparation Equipment

Specimen Preparation Equipment

Inquire with SRNML staff about the availability and use of the following equipment:

General use microscopes

  • A Nikon stereo dissecting microscope with light pipes
  • Two Bausch and Lomb stereo dissecting microscopes
  • Nikon phase contrast laboratory microscope
  • Nikon research photomicroscope with Nomarski interference contrast

Specimen pre-treatment and coating

  • Sputter coating: Anatech Hummer VI
  • Sputter coating: Emitech K575XD
  • Evaporator: JEOL JEE-4C
  • Critical point dryer: Tousimis autosamdri-814

Cryo-TEM

  • GloQube glow discharge
  • cryoplunger Leica GP-2 (located at Stephenson Life Sciences Research Center)
  • Fischione 2550 side-entry cryotransfer holder for JEOL 2010F
  • Pfeiffer HiCube cryopump station
  • Gatan CryoTEM transfer device (for the JEOL 2000FX)
  • Reichert MM-80 contact freezing device

Materials SEM/TEM prep

Biological prep, embedding, and sectioning

  • Three Sorvall MT-2B ultramicrotomes
  • RMC MT-6000 ultramicrotome equipped with FS-1000 cryo-attachment

Equipment

Fischione Model 1051 Ion Mill

Fischione Model 1051 Ion Mill.
Fischione Model 1051 Ion mill equipped with high resolution camera.
pdf Fischione Model 1051 Instructions (PDF)
Operation Instruction for the Fischione Model 1051 Ion Mill.

Fischione Model 200 Dimpling Grinder

Fischione Model 200 Dimpling Grinder.
Fischione Model 200 Dimpling Grinder.

Gatan Model 600 DIFF Ion Mill

Gatan Model 600 DIFF Ion Mill.
Gatan Model 600 DIFF Ion mill.

Allied MultiPrep Polishing Wheel

Allied MultiPrep Polishing Wheel.
Allied MultiPrep Polishing Wheel.