Prices effective July 1st 2020 and are subject to change. Please contact SRNML personnel with any questions or concerns.
Costs for Using SRNML: All users must supply all disposable materials needed for their microscopy work; we endeavor to keep common supplies at-hand for use. A list of those items available though the SRNML can be obtained by consultation with SRNML personnel.
Charges for use (hourly unless otherwise noted) | Internal unassisted | Internal Assisted | External Acad./ Gov | Commercial | |
---|---|---|---|---|---|
Scanning Electron Microscopy | |||||
Tabletop SEM (Hitachi TM-3000) | $25.00 | $35.00 | $54.25 | $89.25 | |
Conventional SEM (Quanta) | $25.00 | $35.00 | $54.25 | $89.25 | |
High resolution FEG-SEM (Zeiss NEON dual-beam) | $45.00 | $65.00 | $100.75 | $165.75 | |
Zeiss Neon after-hours automated runs | $10.00 | $10.00 | $15.50 | $25.50 | |
Quattro S ESEM/EBSD (regular workday use) | $35.00 | $59.75 | $92.61 | $152.36 | |
Quattro S after-hours automated runs | $10.00 | $10.00 | $15.50 | $25.50 | |
Transmission electron microscopy | |||||
Intermediate voltage TEM (JEOL 2000-FX) | $41.60 | $61.60 | $95.48 | $157.08 | |
High resolution FEG-TEM (JEOL 2010F) | $50.00 | $65.00 | $100.75 | $165.75 | |
cryo-TEM (ThermoFisher Tundra) | $50.00 | $85.00 | $131.75 | $216.75 | |
abberation-corrected STEM (coming late 2024) | $50.00 | $85.00 | $131.75 | $216.75 | |
Advanced light microscopy | |||||
Upright CLS/ultiphoto/FLIM microscope (Leica SP8) | $35.00 | $54.25 | $84.09 | $138.34 | |
Leica SP8 special rate for extended XYZT and tiling scans (after first 2 hrs at regular rate) | $15.00 | $23.25 | $36.04 | $59.29 | |
Leica epifluorescence stereo microscope (FM/LM) | $5.00/ $1.35 | $7.75/ $2.09 | $12.01/ $3.24 | $19.76/ $5.33 | |
Keyence VHX-7000 ultramicroscope | no charge | no charge | no charge | $30.00 | |
Zeiss 2000-CS stereomicroscope | no charge | no charge | no charge | $5.00 | |
Zeiss Stemi SV-11 stereomicroscope | no charge | no charge | no charge | $5.00 | |
Preparation costs | |||||
Critical point drying (CO2 costs and cylinder costs) | $23.50 | $23.50 | $36.43 | $59.93 | |
Sputter coating (sputtering head, Argon and cylinder costs) | $8.00 | $12.00 | $18.60 | $30.60 | |
Fischione 1040 NanoMill (per hour) | $8.00 | $15.00 | $23.25 | $38.25 | |
Fischione 1051 ion mill (per sample) | $40.00 | $40.00 | $62.00 | $102.00 | |
Microtomy, (Reichert Ultracut/E) | $12.00 | $20.00 | $31.00 | $51.00 | |
Cryogenics use | |||||
Liquid nitrogen (per liter) | $5.00 | $7.75 | $12.01 | $23.25 | |
X-ray Diffraction | |||||
SmartLab XRD | $15.00 | $25.00 | $38.75 | $63.75 | |
Personnel time | $35.00 | $35.00 | $54.25 | $89.25 | |
Data analysis | |||||
Imaris workstation | $5.70 | $5.70 | $8.84 | $14.54 |
SRNML Director
The SRNML Director is the individual in direct charge of the SRNML and answers directly to the VPRP. Their responsibilities include:
SRNML Research Associates are employees of the SRNML and thus, are supervised by the SRNML Director. Their primary responsibilities are to the shared facility; no individual researcher should expect to receive preferential help from them. Overall responsibilities of Research Associates include:
With respect to point (5) and (6), it is emphasized that researchers who need to use the SRNML on a routine basis should become qualified operators, so they can be responsible for most of their microscopy needs.
SRNML Research Associates may be appointed by the VPRP into Associate Directorship roles, specializing in Advanced Light, Electron, or other microscopy technologies. Associate Directors should work with the SRNML Director to develop and update lab policies, participate in strategic planning, and contribute to teams of researchers working to obtain external support for new microscopy-related research technologies to be included in the SRNML.
The SRNML Steering Committee functions to aid the SRNML Personnel and VPRP in the management of the SRNML. As such, the Steering Committee is asked to make recommendations through the SRNML Director to the VPRP on such matters as:
The Advisory Board will consist of six faculty members appointed by the VPRP in consultation with the SRNML Director and other interested parties. Each term of appointment, subject to one re-appointment, will be for three years. Terms will be staggered such that two new members will be appointed each year. The representation on the Steering Committee will be as broad as possible, including faculty members from a range of departments on campus and regional institutions outside of OU.
Any faculty member, student, post-doctorate, visiting scientist or research staff at the University of Oklahoma may be a user of the SRNML. All users must be trained by SRNML personnel before using facilities and equipment. Trainings and consultations are also available for sample preparation and image analysis. External researchers may be able to perform as an occasional user working with SRNML Research Associates following consultation with the SRNML Director and Research Associates. Users will be considered ‘qualified’ or ‘occasional’.
To become a qualified operator, a researcher must be certified by the SRNML Director that he/she is qualified to operate a particular instrument. Certification must be obtained for each instrument separately. Qualified operators will be classified as follows:
Instrument operators will be assigned to one of the above categories for each instrument by the SRNML Director and Research Associates on the basis of their experience and demonstrated ability to operate the instrument.
In order to train new operators in the use of the instruments and other facilities of the SRNML, the SRNML personnel and associated faculty will offer instruction and training of various types that are designed to meet the varied needs of potential users of the SRNML. The types of instruction will include (but will not be limited to) the following:
Until certified as a qualified operator, a user should not attempt to personally use a microscope unless they are taking a training course and is being directly supervised by SRNML personnel. Use by a non-qualified operator will result in loss of use of the facility.
A qualified operator, who is observed by SRNML personnel not following proper protocol, not correctly using the microscope or using a microscope for which they have not been certified, or not storing/labeling/cleaning their materials adequately will have their qualified status revoked. If the user wants reinstatement as a qualified operator, they must again be certified as qualified.
The regular hours of the SRNML will be from 8:00 a.m. to 5:00 p.m. Monday through Friday (7:30 a.m. to 4:30 p.m., if the University is on that summer schedule). The facility will be open at other times only to qualified day/night operators or by special arrangement with SRNML personnel.
Instrument scheduling: Qualified users will be granted access to online scheduling calendars; all other scheduling will be done by users contacting SRNML personnel. If an SRNML Research Associate is needed to conduct a researcher's microscopy, the researcher should contact them a week or two (the earlier the better) prior to needing the microscopy work. At that time, the SRNML technician can determine their availability, how much time will be needed, and block out these times on the online calendar. If scheduled maintenance/repair or if time for training is needed on an instrument, time will be blocked off on the sign-up sheet by SRNML technicians. The SRNML reserves the right to preempt normal use for maintenance/repair, installations or special circumstances which cannot be successfully predicted and scheduled. Note that electron and advanced light microscopes may have different scheduling policies; refer to the specific instructions for the lab in use. Furthermore, SRNML personnel reserve the right to limit the scheduling of individual researchers who reserve several blocks of time in a single week to allow a range of researchers access to equipment.
No-shows: Anyone scheduled to use the SRNML facilities is expected to do so at the assigned time. If a person repeatedly does not use the microscope at an assigned time, privileges for use of the SRNML facilities will be restricted to open blocks of time during the week the microscopy is to be accomplished.
External usage: External usage of the SRNML is restricted to academic and non-academic projects requiring microscopic services for the purpose of conducting original research. Prices for external usage will be based on all costs incurred by the SRNML in the operation of the facility, including: (1) direct usage and specimen preparation costs; (2) maintenance costs; (3) amortization of research equipment and facilities; (4) operator salaries; (5) fringe benefits; (6) administrative costs; and (7) indirect costs.
Log Books: Log books are provided for each piece of equipment in the SRNML. Each time a piece of equipment is utilized an entry must be placed in the log book. Entries will vary, but will include the name of the user, nature of use, date, and time (starting and finishing) of use. It is essential that these logs be maintained to facilitate instrument maintenance and to determine amount of use each instrument receives.
All SRNML activities are governed by the University lab manual. Users should refer to the online OU Enivronmental Health and Safety Office (ESHO) Lab Manual. The following policies for the most part reiterate key aspects of the OU policies that are most relevant to working in the SRNML. Please refer to the OU Enivronmental Health and Safety Office website for additional information.
The emergency phone number is 911. Mobile phone numbers for SRNML Personnel are listed on each lab door; users are welcome to call.
For safe use of the lab, users should assume they are not allowed to operate or use any equipment until trained. Descriptions of training are provided elsewhere. Furthermore, all users should be current on their laboratory safety, hazard communication, etc. trainings through onpoint.ou.edu. SRNML personnel should provide users a lab/safety orientation for any spaces in the SRNML that the user will be working.
As a multi-user facility, safe and efficient operation requires that all users leave the lab in the same or better condition in which they found it. Return borrowed items to their proper storage location, dispose of waste, clean any used countertops, and return equipment to default settings. Failure to be a good lab citizen may result in loss of access/use privileges.
The SRNML is not responsible for your personal items, specimens, or data. Any items kept in the lab facilities should be labelled with your name. Permission must be requested before storing materials, chemicals, or specimens in lab facilities. SRNML personnel reserve the right to remove or dispose of materials or data in lab facilities.
Food and drink are not allowed outside of offices and conference rooms.
Users must declare all hazardous or infectious materials brought to the lab. The SRNML is not equipped to work with infection materials unless previously fixed and inactivated. Users are responsible for removal and safe disposal of all hazardous materials when the work is complete.
SRNML users and lab personnel work with many hazardous and non-hazardous chemicals.
Start with the assumption that any chemical you encounter is hazardous.
Any chemicals or specimen containers brought to the lab by users must be labelled with the user’s name, contents, and date.
Wear appropriate personal protective equipment (PPE) when working with chemicals, particularly gloves and eye protection.
Volatile chemicals should be used only in a fume hood.
Notify SRNML personnel immediately of any spills or ask questions when uncertain of appropriate procedures.
Note that many of the fixation and staining chemicals contain hazardous materials; treat materials with respect and care. Clean after you work appropriately.
Liquid nitrogen use in SRNML facilities is routine, but care should be taken. Eye protection along with appropriate hand, leg, and foot protection must be worn when transfers of liquid nitrogen; cryo gloves are also available. Note that contact with skin can cause burns, can boil explosively, or displace room air/oxygen. Only store and transfer liquid nitrogen or other cryogens in approved designated containers and use only with approval. Never leave containers containing cryogens unsealed or unattended.
Normal use of SRNML facilities will not result in radiation exposure. However, electron microscopes in particular generate ionizing radiation. Internal shielding precludes the need to use personal protection or monitoring. On the other hand, it is important that you do not use equipment outside the bounds of operating procedures for which you were trained. Radiation safety surveys are performed to ensure safe use. Additionally, uranium-based solutions are sometimes used as part of sample preparation and staining. Such concentrations are safe when used appropriately, but exposure should be minimized and appropriate PPE utilized. Please ask regarding any questions or concerns. You have the right to contact University and State radiation safety officials anytime.
Use of microscopes with lasers requires special operator and laser safety training in consultation with SRNML staff; hazards vary and are specific to each microscope. As with all equipment, use only after training and only in the manner to which the training has been completed.