Protocols
SOPs
Acids (pdf)
Anaerobic Glove Bag (pdf)
Autoclaves (pdf)
Biological Safety Cabinet (pdf)
Bunsen and Ethanol Burners (pdf)
Centrifuges (pdf)
Chloroform (pdf)
Compressed Gases (pdf)
Dishwashing Protocol (pdf)
Dry Ice (pdf)
Freezers (pdf)
Acids (pdf)
Anaerobic Glove Bag (pdf)
Autoclaves (pdf)
Biological Safety Cabinet (pdf)
Centrifuges (pdf)
Chloroform (pdf)
Compressed Gases (pdf)
Dishwashing Protocol (pdf)
Dry Ice (pdf)
Acids (pdf)
Anaerobic Glove Bag (pdf)
Autoclaves (pdf)
Biological Safety Cabinet (pdf)
Centrifuges (pdf)
Chloroform (pdf)
Compressed Gases (pdf)
Dishwashing Protocol (pdf)
Dry Ice (pdf)
Acids (pdf)
Anaerobic Glove Bag (pdf)
Autoclaves (pdf)
Biological Safety Cabinet (pdf)
Centrifuges (pdf)
Chloroform (pdf)
Compressed Gases (pdf)
Dishwashing Protocol (pdf)
Dry Ice (pdf)
Fume Hood (pdf)
Gel Electrophoresis (pdf)
Hazardous Waste Disposal (pdf)
Liquid Nitrogen (pdf)
Phenol (pdf)
Soil and Sample Storage Protocol (pdf)
Spill Kit (pdf)
Transporting Chemicals (pdf)
UV Lights (pdf)
Working with Chemicals (pdf)
Nucleic Acid Extraction Methods
DNeasy PowerSoil DNA Isolation kit (Link to document on the Qiagen website) Nov 2020
DNA Extraction Method for Radioactive sample from ORNL (pdf) May 2017
Modified MoBio PowerSoil ProtocolCombines the freeze-grind DNA extraction method with the MoBio PowerSoil kit
Classic Community DNA extraction methodInstructions for freeze-grind, detergent lysis community DNA extraction - Oct. 2015
Measurement of DNA/RNA
Amplification Methods
Sequencing
Hybridization
Other Assays
Soil PFLA Extraction (pdf)
Chemical and Buffer Preparation
Gel Loading Buffer Recipe (pdf)
TAE Buffer Recipe (pdf)
Phenol Saturation Protocol (pdf) - Instructions for preparing saturated phenol from solid phenol
Instrument Operation
BioScreen (pdf)
EcoPlate (pdf)
NanoDrop One (pdf)
pH Meter (pdf)
Respirometer Protocol (pdf)
Vacuum Manifold (pdf)
