Protein Production and Characterization Core

The purpose of the OU Protein Production and Characterization Core (PPCC) Facility is to aid researchers with customized and comprehensive solutions for protein expression and purification as well as biophysical characterization. We are committed to providing high quality and cost-effective services to accelerate your research at any stage of your project.

Our primary function is protein production for multiple applications such as structural characterization, proteomics and endotoxin free samples for cell-based assays. Our services are available to all protein researchers in any field and we stand ready to assist you. Please feel free to contact us to explore what we can do for you.

Your satisfaction is our goal. Each service we provide is tailor-made to your specific needs. We understand how important timelines are, so we will work with you to establish and meet realistic timelines for each of your projects. You will be kept up-to-date on every project milestone, allowing you full control of the direction your research takes. At the same time, we understand that not all projects produce the desired outcomes. We aim to communicate this realistic perspective to you as soon as a project loses viability, so as to be economically efficient with your budget.

We currently offer the following services:

• Access to and training on laboratory equipment appropriate for protein purification and biophysical characterization
• Small-scale scouting for protein expression profiles
• Protein purification method development
• Protein production (milligram quantities, high purity for structural determination)
• Services in and support for biophysical characterization
• Full, customized protocol creation
• Research consultation

Euan Parnell, PhD, Feinberg School of Medicine, Northwestern University, Chicago, IL: “I have used the PPCC for 3 years, requesting around five separate E.coli purifications. These contracts have been completed in a timely manner with an exceptionally high quality and care - the QC, speed and quality of protein has enabled me to rapidly progress my structural, functional and screening assays, greatly supporting my research and throughput.”

Kara De Leόn, PhD, School of Biological Sciences, University of Oklahoma: “The PPCC has been instrumental in our efforts to purify our proteins. The core director has trained my graduate student in protein isolation methods and has been extremely helpful in troubleshooting the purification process.”

Ravit Boger, MD, Chief, Pediatric Infectious Diseases, Professor of Pediatrics, Microbiology, and Immunology, Medical College of Wisconsin, Milwaukee, WI: “We have been using the protein purification services of the PPCC for a couple of years and are extremely satisfied with the quality and quantity of the purified proteins. Dr. Bourne has been very helpful, knowledgeable, and great to work with. The proteins generated in mammalian cells allowed us to begin identifying epitopes for antibody binding to our protein of interest. I highly recommend this core facility.”