Academics

This workshop focuses on the BioProfile®FLEX2, an essential tool for in-process control (IPC) and monitoring of cell culture parameters during upstream production of biologics in the biomanufacturing industry. Participants will gain practical experience with the hardware and software, learning how to run, analyze, and validate samples for in-process control. This training equips attendees with foundational skills in biomanufacturing analytics, preparing them to confidently support quality and efficiency in biologics production.

Key take aways include: 

• The hardware components of the BioProfile®FLEX2 covering sampling (Syringes, 96-well plate and sample cups), microsensor cards and reagent cartridges.
• Software introduction using the touch screen interface for analyzer operation overview, general settings and test parameter settings
• System operation including loading reagent cartridges, performing calibration and quality control (QC) checks
• The full 16 cell culture test profile including Gluc, Glu, Lac, Gln, NH4+, K+, Na+, Ca++, pH, PO2, PCO2, viable cell density, total cell density, viability, cell diameter and osmolality.
• Loading samples, perform sample analysis and validate test results 
• Performing on-board and external QC, scheduling automatic QC and system maintenance
• Data import/export, results analysis and backing up the database
• Best practices for handling the gas and chemistry microsensor cards, reagent cartridges and system maintenance/shutdown procedure to ensure safe and efficient system operation

This workshop equips participants with the skills to confidently operate  Cedex® Bio Analyzer, an essential analytical instrument for metabolite and product quantification in the biomanufacturing industry during upstream production of therapeutics and bio-based products. Learners will gain hands-on experience with system setup, software operation, and sample validation. The training emphasizes anayalytical techniques for measuring protein and monoclonal antibody concentrations, as well as key metabolites, supporting quality control in both mammalian cell culture and microbial fermentation workflows. Completion of this workshop prepares participants to confidently apply these tools in real-world bioprocessing environments.

Key take aways include: 

• The hardware components of the Cedex Bio covering sample area, fluid system, probe, reagent disk, cuvettes, transfer unit including consumables required for operation
• Software overview for system operation, creating analysis order, fluid handling and administration tasks
• System operation including maintenance, introducing specific test kits, performing calibration and quality control checks.
• Loading samples, perform measurements and validate results for product titer and metabolites
• Data import/export, results viewer software and data management
• Best practice for pre/post run cleaning and maintaining sterility to ensure safe and efficient system operation

Learn the principles and hands-on operation of column chromatography using the ÄKTA avant™ 150 system. This workshop combines lectures, demonstrations, and practical lab sessions to give participants both conceptual understanding and real-world experience in protein purification. Explore various chromatography modes including affinity, ion exchange, and size-exclusion, while gaining proficiency in UNICORN software for method design, monitoring, and data analysis.

Key take aways include: 

• Explain the fundamental principles of column chromatography and its role in protein purification.
• Identify and describe the components of the ÄKTA chromatography system and their functions.
• Prepare samples and buffers appropriately for chromatography experiments using the ÄKTA system.
• Set up and operate the ÄKTA system to perform common chromatography techniques such as affinity chromatography.
• Design and execute affinity chromatography run with optimized parameters for target protein separation and purification.
• Monitor and interpret real-time chromatography data, such as UV absorbance and conductivity, to assess purification progress and quality.
• Troubleshoot common issues encountered during chromatography runs on the ÄKTA system.
• Implement best practices for cleaning, maintenance, and safety when using the ÄKTA chromatography system.
• Analyze and document chromatography results, including yield, purity, and resolution using nanodrop/Cedex Bio analyzers, to be used in the summary report.